教师简介

姓名：	陈忠周
性别：	男
系别：	生物化学与分子生物学
职称：	教授
学位：	博士

I, Zhongzhou Chen, was born in 1974. I majored in protein crystallography.
The structure jmjd2a is the first solved structure of a histone demethylase which has enzymatic activity.
Recently, we solved more than ten structures of the important protein structures,such as histone demethylases, RNA demethylases, abscisic acid receptors and so on.
I published papers on Cell, Genes & Development, Nature Comm., PNAS, Cell Research, Nucleic Acids Research, JBC, Structure, Acta Crystallogr D Biol Crystallogr., JOC, JPCA and so on.

陈忠周 1974年生，教授，博士生导师。入选2007年教育部“新世纪优秀人才支持计划”。2012 年获得国家自然基金优秀青年科学基金项目资助
　　利用X-射线方法研究具有重要生物学意义的蛋白质的立体结构，借助于蛋白质表达、纯化和晶体学以及各种生物物理和生物化学手段分析蛋白质结构和功能关系。从分子水平分析和了解表观遗传或其它重要生命过程中的分子机理和结构基础，从而了解生命活动的本质和解决所面临的各种问题。如所解的JMJD2A结构是世界上首个具有活性中心的组蛋白去甲基化酶家族的结构以及解出JMJD2A复合物、PHF8和LSD2复合物等结构阐述了组蛋白去甲基化酶是如何选择性地催化组蛋白底物。迄今,发表的较高质量的SCI论文已有30多篇,以第一作者或通讯作者在Cell, Genes &Development, Nature Comm., PNAS, Cell Research, Nucleic Acids Research, JBC,Structure, Acta Crystallogr D Biol Crystallogr.等和化学著名的期刊JOC, JPCA等发表多篇高质量论文。目前主持的项目主要有973、自然科学基金面上及优青、教育部新世纪优秀人才、教育部博士点基金等。

学习与工作经历

Working Experience:
2002—2007 Research Associate in Dept. of Immunology
National Jewish Medical and Research Center, USA
2007—Present Professor in College of Biological Sciences
Interest: Protein crystallography
Education:
1997—2002 Ph. D. School of biological science,
Tsinghua University, China
1992—1997 Bachelor of Science, Dept. of Chem.
Tsinghua University, China
1994—1997 Bachelor of Economics Enterprise
Management,College of Economics and Management, Tsinghua University

1992—1997 清华大学 化学系物理化学及仪器分析专业理学学士
1994—1997 清华大学 经济管理学院企业管理专业理学学士
1997—2002 清华大学生命科学院化学生物学专业 理学博士
2002—2007 美国国立犹太人医学研究中心免疫系
2007—目前中国农业大学生物学院(全职)

研究方向

蛋白质晶体学，结构生物学，植物生理学，病毒学，肿瘤学

主讲课程

《Introduction to structure biology》（ 《结构生物学概论》）
《molecular biology》（《分子生物学》）
《高级生物化学与分子生物学 seminar》
《蛋白质表达与纯化技巧》

联系方式

电话：62734078
邮件：chenzhongzhou at cau.edu.cn (please omit the middle space when send email)
通讯地址：北京市海淀区圆明园西路2号生命科学研究中心2071房间

发表文章

发表文章 (*通讯作者，#共同第一作者)

1. Lulu Ma, Hongyun Lu, Zizi Tian, Meiting Yang, Jun Ma, Guohui Shang, Yunlong Liu, Mengjia Xie, Guoguo Wang, Wei Wu, Ziding Zhang, Shaodong Dai, Zhongzhou Chen*. Structural insights into the interactions and epigenetic functions of human nucleic acid repair protein ALKBH6. Journal of Biological Chemistry, 2022, http://doi.org/10.1016/j.jbc.2022.101671

2. Xiaofei Dong, Xue Wang, Mengjia Xie, Wei Wu, Zhongzhou Chen*. Structural Basis of Human Parainfluenza Virus 3 Unassembled Nucleoprotein in Complex with Its Viral Chaperone. Journal of Virology, 2022, 96:e01648-21.

3. Xue Wang, Cuixia Hu, Wei Ye, Jia Wang, Xiaofei Dong, Jie Xu, Xiaorong Li, Manfeng Zhang, Hongyun Lu, Fanglin Zhang, Wei Wu, Shaodong Dai, Hong-Wei Wang*, Zhongzhou Chen*. Structure of Rift Valley fever virus RNA-dependent RNA polymerase, Journal of Virology, 2022, 96: e0171321.

4. Jun Ma, Yiyun Chen, Wei Wu, Zhongzhou Chen*. Structure and Function of N-Terminal Zinc Finger Domain of SARS-CoV-2 NSP2. Virologica Sinica. 2021, 36:1104-1112.

5. Guoguo Wang, Mengjia Xie, Wei Wu, Zhongzhou Chen*. Structures and Functional Diversities of ASFV Proteins. Viruses, 2021, 13, 2124. 

6. Jun Ma, Lulu Ma, Meiting Yang, Wei Wu, Wenhai Feng, Zhongzhou Chen*. The Function of the PRRSV–Host Interactions and Their Effects on Viral Replication and Propagation in Antiviral Strategies. Vaccines (Basel), 2021, 9:364.

7. Hongyun Lu, Yumeng Zhan, Xiaorong Li, Xuehui Bai, Feifei Yuan, Lulu Ma, Xue Wang, Mengjia Xie, Wei Wu, Zhongzhou Chen*. Novel insights into the function of an N-terminal region of DENV2 NS4B for the optimal helicase activity of NS3. Virus Research, 2021, 295:198318

8. Haolin Liu, Qianqian Zhang, Pengcheng Wei , Zhongzhou Chen, Katja Aviszus , John Yang, Walter Downing, Chengyu Jiang, Bo Liang, Lyndon Reynoso, Gregory P Downey, Stephen K Frankel, John Kapple , Philippa Marrack, Gongyi Zhang. The basis of a more contagious 501Y.V1 variant of SARS-CoV-2. Cell Research, 2021, 31:720-722.

9. 李智慧，商国辉，唐晨骏，田姿姿，吴玮，陈忠周*。人泛素连接酶AREL1催化结构域的晶体结构. 生物化学与生物物理进展，2020, 4:335-343.

10. Haolin Liu, Srinivas Ramachandran, Nova Fong, Tzu Phang, Schuyler Lee, Pirooz Parsa, Xinjian Liu, Laura Harmacek, Thomas Danhorn, Tengyao Song, Sangphil Oh, Qianqian Zhang, Zhongzhou Chen, Qian Zhang, Ting-Hui Tu, Carrie Happoldt, Brian O’Conner, Ralf Janknecht, Chuan-Yuan Li, Philippa Marrack, John Kappler, Sonia Leach, and Gongyi Zhang. JMJD5 couples with CDK9 to release the paused RNA polymerase II. Proc Natl Acad Sci U S A, 2020, 117:19888-19895.

11. Zizi Tian, Xiaorong Li, Min Li, WeiWu, Manfeng Zhang, Chenjun Tang, Zhihui Li, Yunlong Liu, Zhenhang Chen, Meiting Yang, Lulu Ma, Cody Caba, Yufeng Tong, Hon-Ming Lam, Shaodong Dai, Zhongzhou Chen*. Crystal structures of REF6 and its complex with DNA reveal diverse recognition mechanisms. Cell Discovery, 2020, 6, 17.

12. Chenjun Tang, Zengqin Deng, Xiaorong Li, Meiting Yang, Zizi Tian, Zhenhang Chen, Guoguo Wang, WeiWu, Wen-hai Feng, Gongyi Zhang, Zhongzhou Chen*. Helicase of Type 2 Porcine Reproductive and Respiratory Syndrome Virus Strain HV Reveals a Unique Structure. Viruses. 2020, 12:215.

13. Schuyler Lee, Haolin Liu, Ryan Hill, Chunjing Chen, Xia Hong, Fran Crawford, Molly Kingsley, Qianqian Zhang, Xinjian Liu, Zhongzhou Chen, Andreas Lengeling, Kathrin Maria Bernt, Philippa Marrack, John Kappler, Qiang Zhou, Chuan-Yuan Li, Yuhua Xue, Kirk Hansen, Gongyi Zhang. JMJD6 cleaves MePCE to release positive transcription elongation factor b (P-TEFb) in higher eukaryotes. Elife, 2020, 9:e53930.

14. Li-Fei Tian, Yan-Ping Liu, Lianqi Chen, Qun Tang, Wei Wu, Wei Sun, Zhongzhou Chen*, Xiao-Xue Yan*. Structural basis of nucleic acid recognition and 6mA demethylation by human ALKBH1. Cell Research, 2020, 30:272-275.

15. Jimin Yuan, Wan Hwa Ng, Zizi Tian, Jiajun Yap, Manuela Baccarini, Zhongzhou Chen, Jiancheng Hu. Activating mutations in MEK1 enhance homodimerization and promote tumorigenesis. Sci Signal. 2018, 11: eaar6795.

16. Wenli Tian, Min Li, Huiyuan Guo, Wenjun Peng, Xiaofeng Xue, Yifan Hu, Yang Liu, Yazhou Zhao, Xiaoming Fang, Kai Wang, Xiuting Li, Yufeng Tong, Michael A. Conlon, Wei Wu, Fazheng Ren, Zhongzhou Chen*. Architecture of the Native Major Royal Jelly Protein 1 Oligomer. Nature Comm, 2018, 9:3373.

17. Haolin Liu, Chao Wang, Schuyler Lee, Yu Deng, Matthew Wither, Sangphil Oh, Fangkun Ning, Carissa Dege, Qianqian Zhang, Xinjian Liu, Aaron M. Johnson, Jianye Zang, Zhongzhou Chen, Ralf Janknecht, Kirk Hansen, Philippa Marrack, Chuan-Yuan Li, John W. Kappler, James Hagman, Gongyi Zhang. Clipping of arginine-methylated histone tails by JMJD5 and JMJD7. Proc Natl Acad Sci U S A, 2017, 114:E7717-E7726.

18. Wei Wei, Xiaoguang Liu, Jiwei Chen, Shennan Gao, Lu Lu, Huifang Zhang, Guangjin Ding, Zhiqiang Wang, Zhongzhou Chen, Tieliu Shi, Jiwen Li, Jianjun Yu, Jiemin Wong. Class I histone deacetylases are major histone decrotonylases: evidence for critical and broad function of histone crotonylation in transcription. Cell Research, 2017, 27:898–915.

19. Xiaoqiang Han, Lun Jiang, Chuanliang Che, Chuan Wan, Huizhe Lu, Yumei Xiao, Yanjun Xu, Zhongzhou Chen*, Zhaohai Qin*. Design and functional characterization of a novel abscisic acid analog. Scientific Reports, 2017, 7: 43863.

20. Yina Gao, Qi Zhang, Yue Lang, Yang Liu, Xiaofei Dong, Zhenhang Chen, Wenli Tian, Jun Tang, Wei Wu, Yufeng Tong, Zhongzhou Chen*. Human apo-SRP72 and SRP68/72 complex structures reveal the molecular basis of protein translocation. Journal of Molecular Cell Biology, 2017, 9 (3): 220-230.

21. Manfeng Zhang, Xiaorong Li, Zengqin Deng, Zhenhang Chen, Yang Liu, Yina Gao, Wei Wu, Zhongzhou Chen*. Structural Biology of the Arterivirus nsp11 Endoribonucleases. J Virol, 2017, 91: e01309-16.

22. Ming-Yan Cheung, Xiaorong Li, Rui Miao, Yu-Hang Fong, Kwan-Pok Li, Yuk-Lin Yung, Mei-Hui Yu, Kam-Bo Wong^*, Zhongzhou Chen^*, Hon-Ming Lam^*. ATP binding by the P-loop NTPase OsYchF1 (an unconventional G protein) contributes to biotic but not abiotic stress responses. Proc Natl Acad Sci U S A, 2016, 113:2648-53.

23. Lun Jiang, Yibo Chen, Jiangge Zheng, Zhenhang Chen, Yujie Liu, Ye Tao, Wei Wu, Zhongzhou Chen^*, Baichen Wang*. Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein. Plant Physiology. 2016, 170:732-41.

24. Yue Xing, Yufeng Hu, Lun Jiang, Zideng Gao, Zhenhang Chen, Zhongzhou Chen*, Xueqin Ren*. Zwitterion-Immobilized Imprinted Polymers for Promoting the Crystallization of Proteins. Cryst. Growth Des. 2015, 15, 4932–4937.

25. Jiangge Zheng, Yue Lang, Qi Zhang, Di Cui, Haili Sun, Lun Jiang, Zhenhang Chen, Rui Zhang, Yina Gao, Wenli Tian, Wei Wu, Jun Tang*, Zhongzhou Chen*. Structure of human MDM2 complexed with RPL11 reveals the molecular basis of p53 activation. Genes & Development. 2015, 29:1524-34.

26. Yufeng Hu, Zhenhang Chen, Yanjun Fu, Qingzhong He, Lun Jiang, Jiangge Zheng, Yina Gao, Pinchao Mei, Zhongzhou Chen*, Xueqin Ren*. The amino-terminal structure of human Fragile X Mental Retardation Protein obtained using precipitant-immobilized imprinted polymers. Nature Comm. 2015, 6:6634.

27. Zengqin Deng, Qing Wang, Zhao Liu, Manfeng Zhang, Ana Carolina Dantas Machado, Tsu-Pei Chiu, Chong Feng, Qi Zhang, Lin Yu, Lei Qi, Jiangge Zheng, Xu Wang, XinMei Huo, Xiaoxuan Qi, Xiaorong Li, Wei Wu, Remo Rohs*, Ying Li*, Zhongzhou Chen*. Mechanistic insights into metal ion activation and operator recognition by the ferric uptake regulator. Nature Comm. 2015, 6:7642.

28. Jing Li, Juntao Liu, Guoqiang Wang, Joon-Yung Cha, Guannan Li, She Chen, Zhen Li, Jinghua Guo, Caiguo Zhang, Yongqing Yang, Woe-Yeon Kim, Dae-Jin Yun, Karen S. Schumaker, Zhongzhou Chen, Yan Guo. A Chaperone Function of NO CATALASE ACTIVITY 1 Is Required to Maintain Catalase Activity and for Multiple Stress Responses in Arabidopsis. Plant Cell. 2015, 27:908-25.

29. Yusheng Zhao, Shaojun Xie, Xiaojie Li, Chunlei Wang, Zhongzhou Chen, Jinsheng Lai,  Zhizhong Gong. REPRESSOR OF SILENCING 5 encodes a member of the small heat shock protein family and is required for DNA demethylation in Arabidopsis. Plant Cell. 2014, 26:2660-2675.

30. Xingliang Zhang*, Lun Jiang, Qi Xin, Yang Liu, Jianxin Tan, Zhongzhou Chen*. Structural Basis and Functions of Abscisic Acid Receptors PYLs. Frontiers in Plant Science. 2015, 6:88.

31. Jiangge Zheng, Junru Li, Lei Han, Yang Wang, Wei Wu, Xiaoxuan Qi, Ye Tao, Long Zhang, Ziding Zhang, Zhongzhou Chen*. Crystal structure of the Locusta migratoria odorant binding protein. Biochem Biophys Res Commun. 2015, 456:737-42.

32. Chao Wang#, Zhongzhou Chen#, Xia Hong, Fangkun Ning, Haolin Liu, Jianye Zang, Xiaoxue Yan, Jennifer Kemp, Catherine Musselman, Tatinna Kutateladze, Rui Zhao, Chengyu Jiang*, Gongyi Zhang*. The structural basis of urea-induced protein unfolding in beta-catenin. Acta Crystallogr D Biol Crystallogr. 2014, 70:2840-2847.

33. Guoqiang Wang, Qingzhong He, Chong Feng, Yang Liu, Zengqin Deng, Xiaoxuan Qi, Wei Wu, Pinchao Mei, Zhongzhou Chen*. The atomic-resolution structure of human alkb homolog 7 (ALKBH7), a key protein for programmed necrosis and fat metabolism. J Biol Chem. 2014, 289:27924-36.

34. Chong Feng, Yang Liu, Guoqiang Wang, Zengqin Deng, Qi Zhang, Wei Wu, Yufeng Tong, Changmei Cheng*, Zhongzhou Chen*. Crystal structures of the human rna demethylase Alkbh5 reveal basis for substrate recognition. J Biol Chem, 2014, 289:11571-83

35. Zengqin Deng, Kathleen C. Lehmann, Xiaorong Li, Chong Feng, Guoqiang Wang,Qi Zhang, Xiaoxuan Qi, Lin Yu, Xingliang Zhang, Wenhai Feng, Wei Wu, Peng Gong, Ye Tao, Clara C. Posthuma, Eric J. Snijder, Alexander E. Gorbalenya*, Zhongzhou Chen*. Structural basis for the regulatory function of a complex zinc-binding domain in a replicative arterivirus helicase resembling a nonsense-mediated mRNA decay helicase. Nucleic Acids Research, 2014, 42:3464-77.

36. Xingliang Zhang, Lun Jiang, Guoqiang Wang, Lin Yu, Qi Zhang, Qi Xin, Wei Wu , Zhizhong Gong, Zhongzhou Chen*. Structural insights into the abscisic acid stereospecificity by the ABA receptors PYR/PYL/RCAR. PLoS One, 2013, 8:e67477.

37. Wenli Tian, Lingyu Gao, Yazhou Zhao, Wenjun Peng* and Zhongzhou Chen*. Simultaneous determination of metronidazole, chloramphenicol and 10 sulfonamide residues in honey by LC–MS/MS. Anal. Methods, 2013, 5:1283-1288.

38. Qi Zhang, Shankang Qi, Mingchu Xu, Lin Yu, Ye Tao, Zengqin Deng, Wei Wu, Zhongzhou Chen*, Jiemin Wong*. Structure-function analysis reveals a novel mechanism for regulation of histone demethylase LSD2/AOF1/KDM1b. Cell Research, 2013, 23: 225-241.

39. Yunyun Geng, Shamci Monajembashi, Anwen Shao, Di Cui, Weiyong He, Zhongzhou Chen, Peter Hemmerich, Jun Tang. Contribution of the C-terminal regions of promyelocytic leukemia protein (PML) isoforms II and V to PML nuclear body formation. J Biol Chem. 2012, 287: 30729-42.

40. Xingliang Zhang, Qi Zhang, Qi Xin, Lin Yu, Zheng Wang, Wei Wu, Lun Jiang, Guoqiang Wang, Wenli Tian, Zengqin Deng, Yang Wang, Zhao Liu, Jiafu Long, Zhizhong Gong，Zhongzhou Chen*. Complex structures of the abscisic acid receptor PYL3/RCAR13 reveal a unique regulatory mechanism. Structure, 2012, 20:780-790.

41. Lin Yu, Yang Wang, Shuo Huang, Jianjun Wang, Zengqin Deng, Qi Zhang, Wei Wu, Xingliang Zhang, Zhao Liu, Weimin Gong, Zhongzhou Chen*. Structural insights into a novel histone demethylase PHF8. Cell Research, 2010, 20:166-73.

42. Zhongzhou Chen, Jianye Zang, Fei Lan, Xia Hong, Qin Wang, Shaodong Dai, Kirk Hansen, Yang Shi, and Gongyi Zhang. Structural basis of the recognition of a methylated histone tail by JMJD2A. Proc Natl Acad Sci U S A, 2007, 104:10818–10823.

43. Zhongzhou Chen#, Jianye Zang#, Johnathan Whetstine, Xia Hong, Foteini Davrazou, Tatiana G. Kutateladze, Michael Simpson, Qilong Mao, Cheol-Ho Pan, Shaodong Dai, James Hagman, Kirk Hansen, Yang Shi,  Gongyi Zhang. Structural insights into histone demethylation by JMJD2 family members. Cell, 2006, 125:691–702.

44. Johnathan R. Whetstine, Amanda Nottke, Fei Lan, Maite Huarte, Sarit Smolikov, Zhongzhou Chen, Eric Spooner, En Li, Gongyi Zhang, Monica Colaiacovo, Yang Shi. Reversal of histone lysine trimethylation by the JMJD2 family of histone demethylases. Cell, 2006, 125:467–481.

45. Zhong-Zhou Chen, Yan-Mei Li, Hai-Yan Wang, Jin-Tang Du, Yu-Fen Zhao. Theoretical study on the rearrangement of beta-OH and gamma-OH in ESI mass spectrometry by N-phosphorylation. Journal of Physical Chemistry A, 2004, 108:7686-7690.

46. Zhong-Zhou Chen, Bo Tan, Yan-Mei Li, Yu-Fen Zhao, Yu-Feng Tong, Jin-Feng Wang. Activity difference between alpha-COOH and beta-COOH in N-phosphorylaspartic acids. Journal of Organic Chemistry, 2003, 68:4052-4058.

47. Zhong-Zhou Chen, Yan-Mei Li, Jing Ma, Bo Tan, Satoshi Inagaki, Yu-Fen Zhao, Comparing the activities of alpha-COOH and gamma-COOH in N-phosphoryl glutamic acid by theoretical study. Journal of Physical Chemistry A, 2002, 106:11565-11569.

48. Zhong-Zhou Chen, Yan-Mei Li, Xi Peng, Fu-Rong Huang, Yu-Fen Zhao. The Use of crude lipase in deprotection of C-terminal protecting groups. Journal of Molecular Catalysis B: Enzymatic, 2002, 18:243–249.

49. Zhong-Zhou Chen, Shui-Bing Chen, Yi Chen, YanMei Li, Jing Chen, Yu-Fen Zhao. Negative-ion ESI mass spectrometry of N-phosphoryl amino acids and dipeptides. Rapid Commun. Mass Spectrom. 2002, 16:790-796.

50. ZhongZhou Chen, YuFeng Tong, ShuiBing Chen, YanMei Li, Yi Chen, YuFen Zhao, JinFeng Wang. Orientation of the peptide formation of N-phosphoryl amino acids in solution. Chinese Science Bull. 2002, 47:1866-1870.

51. Zhong-Zhou Chen, Bo Tan, Yan-Mei Li, Yu-Fen Zhao. Differentiation of alpha-COOH from beta-COOH in aspartic acids by N-phosphorylation. International Journal of Quantum Chemistry, 2001, 83:41-51.

52. Zhong-Zhou Chen, Bo Tan, Yan-Mei Li, Yi Chen, Chang-Mei Cheng, Yu-Fen Zhao Differentiation of alpha-COOH from gamma-COOH in glutamic acids by N-phosphorylation. Journal of Molecular Structure: THEOCHEM, 2001, 574:163-175.

获奖情况

2012年全国优秀青年基金获得者
2007: 入选 2007年教育部 "新世纪优秀人才支持计划"
2006: NJMRC Vestal Viola Coulter Scholarship
2006: Elected to Sigma Xi
2004: Nomination awards of the national outstanding Doctoral dissertations by the Ministry of Education (全国百篇优秀博士论文提名奖)and First grade outstanding Ph. D. dissertation of Tsinghua University
1992-2002: Various scholarships of Tsinghua University in every year.
Other than these,
1997: Excellent undergraduate Thesis and Honored Graduate of Tsinghua University.
1994: Scholarship from Hongkong alumni of Tsinghua University.
1993: Second Prize - 5th Mathematics contest of undergraduate course(北京市高等数学本科甲组二等奖), Beijing, 1993
1992: Quintessence Student of Fujian Province

承担项目及课题

973, 自然科学基金等

专利情况

社会兼职

美国晶体学协会会员中国晶体学学会终身会员, 中国生物物理学会会员。