教师简介 | 姓名： | 李大伟 | 
| | 性别： | 男 | | 系别： | 微生物学与免疫学 | | 职称： | 教授 | | 学位： | 博士 |
李大伟教授，1964年10月生于河南。1995年于北京农业大学获理学博士学位。2003年度国家杰出青年科学基金获得者。
学习与工作经历 1981.9-1985.7 河南农业大学植保专业 本科生
1985.7-1988.8 河南农业大学植保系 助教
1988.9-1991.7 沈阳农业大学植物病理学专业 硕士研究生
1991.9-1995.3 北京农业大学植物病理学专业 博士研究生
1995.12-1997.11 中国科学院遗传所 博士后
1997.12-2002.12 中国农业大学农业生物技术国家重点实验室 副教授
1999.11-2002.8 美国 University of Colorado Health Sciences Center 生化与分子遗传学系 博士后
2002.12-今 中国农业大学生物学院微生物学与免疫学系及农业生物技术国家重点实验室 教授、博士生导师
2007.3-2007.9 美国Oregon State University的James Carrington教授实验室高级研究学者
研究方向
主要从事分子植物病毒学的基础理论及应用基础研究。近年来以大麦条纹花叶病毒 (Barley
stripe mosaic virus，BSMV) 为研究重点, (1) 分析BSMV编码蛋白之间的相互作用、表达调控及其在病毒复制、移动和致病性中的作用； (2)BSMV编码蛋白的翻译后修饰及其功能分析；(3)BSMV与寄主植物的相互作用的分子机制, 包括关键致病因子及其氨基酸的鉴定、病毒蛋白-寄主蛋白互作的生物学功能分析等。
主讲课程 本科生《病毒学》 研究生《生命科学文献研读（微生物专业）》 研究生《科技论文写作》（主持）
联系方式
电话：010-62733326
邮件：Lidw at cau.edu.cn
通讯地址：北京市海淀区圆明园西路2号,中国农业大学生物学院，邮编：100193
发表文章
Gerda Szakonyi, Joel M Guthridge, Dawei Li, Young Kendra, Michael V Holers, Xiaojiang S Chen, 2001, Structure of complement receptor 2 in complex with its C3d ligand, Science, 292: 1725-1728. Dawei Li, Rui Zhao, Wayne Lilyestrom, Dahai Gai, Rongguang Zhang, DeCaprio James, Ellen Fanning, Gerda Szakonyi, Xiaojiang S Chen, 2003, Structure of the replicative helicase of the oncoprotein SV40 large tumour antigen, Nature, 423: 512-518. Dahai Gai, Dawei Li, Carla V Finkielstein, Robert D Ott, Poonam Taneja, Ellen Fanning, Xiaojiang S Chen, 2004, Insights into the oligomeric states, conformational changes and helicase activities of SV40 large tumor antigen, Journal of Biological Chemistry, 279: 38952-38959. Dahai Gai, Rui Zhao, Dawei Li, Carla V Finkielstein, Xiaojiang S Chen, 2004, Mechanisms of conformational change for a replicative hexameric helicase of SV40 large T antigen, Cell, 119: 47-60. Lihua Guo, Yunhe Cao, Dawei Li, Shengniao Niu, Zhunan Cai, Chenggui Han, Yafeng Zhai, Jialin Yu, 2005, Analysis of nucleotide sequences and multimeric forms of a novel satellite RNA associated with Beet black scorch virus, Journal of Virology, 79: 3664-3674. Yunhe Cao, Xuefeng Yuan, Xiaoxing Wang, Lihua Guo, Zhunan Cai, Chenggui Han, Dawei Li*, Jialin Yu, 2006, Effect on viral pathogenicity of Beet black scorch virus coat protein, Progress in Biochemistry and Biophysics, 33: 127-134. Zhaohui Wang, Shuoguo Fang, Zhiyan Zhang, Chenggui Han, Dawei Li*, Jialin Yu, 2006, Development of an ID-ELISA for the detection of Rice black-streaked dwarf virus in plants, Journal of Virological Methods, 134: 61-65. Xuefeng Yuan, Yunhe Cao, Dehui Xi, Lihua Guo, Chenggui Han, Dawei Li*, Yafeng Zhai, Jialin Yu, 2006, Analysis of the subgenomic RNAs and the small ORFs of Beet black scorch virus by in vitro transcripts, Journal of General Virology, 78: 3077-3086. Dehui Xi, Jiang Li, Chenggui Han, Dawei Li*, Jialin Yu, Xueping Zhou, 2008, Complete nucleotide sequence of a new strain of Tobacco necrosis virus A infecting soybean in China and infectivity of its full-length cDNA clone, Virus Genes, 36: 259-266. Montgomery T A, Howell M, Cuperus J T, Dawei Li, Alexander A, Chapman E J, Fahlgren N, Allen E, and James C Carrington, 2008, Specificity of ARGONAUTE7-miR390 interaction and dual functionality of ARGONAUTE7-miR390 complexes in TAS3 trans-acting siRNA formation, Cell, 133: 128-141. Yongliang Zhang, Jiang Li, Heng Pu, Jin Jin, Xiaofeng Zhang, Mingkuan Chen, Bin Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2010, Development of Tobacco necrosis virus A as a vector for efficient and stable expression of FMDV VP1 peptides, Plant Biotechnology Journal, 8(4): 506-523. Bo Zhou, Yongliang Zhang, Xianbing Wang, Jiangli Dong, Bin Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2010, Oral administration of plant-based rotavirus VP6 induces antigen-specific IgAs, IgGs and passive protection in mice, Vaccine, 28(37): 6021-6027. Yanhong Han, Haiying Xiang, Qian Wang, Yuanyuan Li, Wenqi Wu, Chenggui Han, Dawei Li, Jialin Yu, 2010, Ring structure amino acids affect the suppressor activity of Melon aphid-borne yellows virus P0 protein. Virology, 406(1): 21-27. Yanjing Zhang, Xiaofeng Zhang, Shaofang Niu, Chenggui Han, Jialin Yu, Dawei Li*, 2011, Nuclear localization of Beet black scorch virus capsid protein and its interaction with importin a, Virus Research, 155(1): 307-315. Cheng Yuan+, Cui Li+, Lijie Yan, Andrew O Jackson, Zhiyong Liu, Chenggui Han, Jialin Yu, Dawei Li*, 2011, A high throughput Barley stripe mosaic virus vector for virus induced gene silencing in monocots and dicots, PLoS ONE, 6: e26468. Yu Cui, Mi Yeon Lee, Naxin Huo, Jennifer Bragg, Lijie Yan, Cheng Yuan, Cui Li, Sara J. Holditch, Jingzhong Xie, Ming-Cheng Luo, Dawei Li, Jialin Yu, Joel Martin, Wendy Schackwitz, Yong-Qiang Gu, John P. Vogel, Andrew O. Jackson, Zhiyong Liu, David F. Garvin, 2012, Fine mapping of the Barley stripe mosaic virus resistance gene Bsr1 in the model grass Brachypodium distachyon, PLoS ONE, 7(6): e38333. Xiaohui Wang, Yanjing Zhang, Jin Xu, Lindan Shi, Huiyan Fan, Dawei Li, Chenggui Han, Jialin Yu, 2012, The R-rich motif of Beet black scorch virus P7a movement protein is important for the nuclear localization, nucleolar targeting and viral infectivity, Virus Research, 167(2): 207-218. Jin Xu, Xianbing Wang, Lindan Shi, Yuan Zhou, Dawei Li, Chenggui Han, Ziding Zhang, Jialin Yu, 2012, Two distinct sites are essential for virulent infection and support of variant satellite RNA replication in spontaneous Beet black scorch virus variants. Journal of General Virology, 93(12): 2718-2728. Mi Yeon Lee+, Lijie Yan+, Florien A. Gorter, Brian Y.T. Kim, Yu Cui, Yue Hu, Cheng Yuan, Jessica Grindheim, Uma Ganesan, Zhiyong Liu, Chenggui Han, Jialin Yu, Dawei Li*, Andrew O. Jackson*, 2012, Brachypodium distachyon Bd3-1 resistance is elicited by the Barley stripe mosaic virus Triple Gene Block 1 movement protein. Journal of General Virology, 93(12): 2729-2739. (Cover story) Xiuling Cao, Yingui Lu, Dianping Di, Zhiyan Zhang, He Liu, Lanzhi Tian, Aihong Zhang, Yanjing Zhang, Lindan Shi, Bihong Guo, Jin Xu, Xifei Duan, Xianbing Wang, Chenggui Han, Hongqin Miao*, Jialin Yu*, Dawei Li*, 2013, Enhanced virus resistance in transgenic maize expressing a dsRNA-specific endoribonuclease gene from E. coli. PLoS ONE, 8(4): e60829. Xiaofeng Zhang, Xiaofei Zhao, Yanjing Zhang, Shaofang Niu, Feng Qu, Yongliang Zhang, Chenggui Han, Jialin Yu, Dawei Li*, 2013, N-terminal basic amino acid residues of Beet black scorch virus capsid protein play a critical role in virion assembly and systemic movement, Virology Journal, 10(1): 200. Kun Zhang, Shaofang Niu, Dianping Di, Lindan Shi, Deshui Liu, Xiuling Cao, Hongqin Miao, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li, Yongliang Zhang, 2013, Selection of reference genes for gene expression studies in virus-infected monocots using quantitative real-time PCR. Journal of Biotechnology, 168(1): 7-14. Tao Zhuo, Yuanyuan Li, Haiying Xiang, Zhanyu Wu, Xianbin Wang, Ying Wang, Yongliang Zhang, Dawei Li, Jialin Yu, Chenggui Han, 2014. Amino acid sequence motifs essential for P0-mediated suppression of RNA silencing in an isolate of Potato leafroll virus from Inner Mongolia. Molecular Plant-Microbe Interactions, 27(6): 515-527. Xiuling Cao, Xuejiao Jin, Xiaofeng Zhang, Ying Li, Chunyan Wang, Xianbing Wang, Jian Hong, Xiaofeng Wang, Dawei Li, Yongliang Zhang*, 2015. Morphogenesis of endoplasmic reticulum membrane-invaginated vesicles during Beet black scorch virus infection: role of auxiliary replication protein and new implications of three-dimensional architecture. Journal of Virology, 89(12): 6184-6195. Xiaofei Zhao, Xiaoling Wang, Kai Dong, Yongliang Zhang, Yue Hu, Xin Zhang, Yanmei Chen, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2015, Phosphorylation of Beet black scorch virus coat protein by PKA is required for the assembly and stability of virus particles. Scientific Reports, 5: 11585. Yue Hu+, Zhenggang Li+, Cheng Yuan, Xuejiao Jin, Lijie Yan, Xiaofei Zhao, Yongliang Zhang, Andrew O. Jackson, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2015, Phosphorylation of TGB1 by protein kinase CK2 promotes Barley stripe mosaic virusmovement in monocots and dicots, Journal of Experimental Botany, 66 (15): 4733-4747. Guoxin Wang, Ling Wang, Yu Cui, Meihua Yu, Chen Dang, Hao Wang, Xuejiao Jin, Lijie Yan, Qiuhong Wu, Dawei Li, Zhiyong Liu*, 2017, RNA-seq analysis of Brachypodium distachyon responses to Barley stripe mosaic virus infection, The Crop Journal, 5(1): 1-10. Kun Zhang+, Yongliang Zhang+, Meng Yang, Songyu Liu, Zhenggang Li, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2017, The Barley stripe mosaic virus γb protein promotes chloroplast-targeted replication by enhancing unwinding of RNA duplexes, PLoS Pathogens, 13(4): e1006319. Michael J. Adams, Scott Adkins, Claude Bragard, David Gilmer, Dawei Li, Stuart A. MacFarlane, Sek-Man Wong, Ulrich Melcher, Claudio Ratti, Ki Hyun Ryu, ICTV Report Consortium, 2017, ICTV virus taxonomy profile: Virgaviridae. Journal of General Virology, 98(8): 1999-2000. Zhihao Jiang+, Zhaolei Li+, Ning Yue, Kun Zhang, Dawei Li, Yongliang Zhang*, 2018, Construction of infectious clones of Lychnis ringspot virus and evaluation of its relationship with Barley stripe mosaic virus by reassortment of genomic RNA segments, Virus Research, 243: 106-109. Xuejiao Jin, Zhihao Jiang, Kun Zhang, Pengfei Wang, Xiuling Cao, Ning Yue, Xueting Wang, Xuan Zhang, Yunqin Li, Dawei Li, Byung-Ho Kang, Yongliang Zhang*, 2018, Three-dimensional analysis of chloroplast structures associated with virus infection, Plant Physiology, 176(1): 284-294. Xiaoling Wang+, Xiuling Cao+, Min Liu, Ruiqi Zhang, Xin Zhang, Zongyu Gao, Xiaofei Zhao, Kai Xu, Dawei Li, Yongliang Zhang*, 2018, Hsc70-2 is required for Beet black scorch virus infection through interaction with replication and capsid proteins, Scientific Reports, 8: 4526. Meng Yang, Zhenggang Li, Kun Zhang, Xuan Zhang, Yongliang Zhang, Xianbing Wang, Chenggui Han, Jialin Yu, Kai Xu*, Dawei Li*, 2018, Barley stripe mosaic virus γb interacts with glycolate oxidase and inhibits peroxisomal ROS production to facilitate virus infection, Molecular Plant, 11(2): 338-341. Zhenggang Li, Yongliang Zhang, Zhihao Jiang, Xuejiao Jin, Kun Zhang, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2018, Hijacking of the nucleolar protein fibrillarin by TGB1 is required for cell-to-cell movement of Barley stripe mosaic virus, Molecular Plant Pathology, 19(5): 1222-1237 (Recommended in F1000Prime: https://f1000.com/prime/731253032 ). Xuan Zhang, Kai Dong, Kai Xu, Kun Zhang, Xuejiao Jin, Meng Yang, Yongliang Zhang, Xianbing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2018, Barley stripe mosaic virus infection requires PKA-mediated phosphorylation of γb for suppression of both RNA silencing and host cell death response, New Phytologist, 218(4): 1570-1585. Meng Yang, Yongliang Zhang, Xialin Xie, Ning Yue, Xianbing Wang, Chenggui Han, Jialin Yu, Yule Liu*, Dawei Li*, 2018, Barley stripe mosaic virus γb subverts autophagy to promote viral infection by disrupting the ATG7-ATG8 interaction, The Plant Cell, 30 (7): 1582-1595. Jiacheng Hu, Shaoya Li, Zhaolei Li, Huiyuan Li, Weibin Song, Haiming Zhao, Jinsheng Lai, Lanqin Xia, Dawei Li, Yongliang Zhang*, 2019, Barley stripe mosaic virus-based guide RNA delivery system for targeted mutagenesis in wheat and maize, Molecular Plant Pathology, 20(10): 1463-1474. Zhaolei Li+, Zhihao Jiang+, Ning Yue, Xinxin Yang, Xueting Wang, Kun Zhang, Andrew O. Jackson, Dawei Li, Yongliang Zhang*, 2020, Construction of an infectious Poa semilatent virus cDNA clone and comparisons of hordeivirus replication and pathogenicity, Phytopathology, 110(1): 215-227. Zhihao Jiang, Kun Zhang, Zhaolei Li, Zhenggang Li, Meng Yang, Xuejiao Jin, Qing Cao, Xueting Wang, Ning Yue, Dawei Li, Yongliang Zhang*, 2020, The Barley stripe mosaic virus γb protein promotes viral cell-to-cell movement by enhancing ATPase-mediated assembly of ribonucleoprotein movement complexes, PLoS Pathogens, 16(7): e1008709. Zongyu Gao, Heng Pu, Jingyuan Liu, Xiaoling Wang, Chenchen Zhong, Ning Yue, Ziding Zhang, Xian-bing Wang, Chenggui Han, Jialin Yu, Dawei Li, Yongliang Zhang*, 2021, Single amino acid in the coat protein determines host-specific systemic infectivity of Tobacco necrosis virus-AC in Nicotiana benthamiana and soybean, Molecular Plant-Microbe Interactions, 34(1): 49-61. Xueting Wang, Juncai Ma, Xuejiao Jin, Ning Yue, Peng Gao, Keith Ka Ki Mai, Xian-Bing Wang, Dawei Li, Byung-Ho Kang*, Yongliang Zhang*, 2021, Three-dimensional reconstruction and comparison of vacuolar membranes associated with virus infections, Journal of Integrative Plant Biology, 63(2): 353-364. Zhihao Jiang, Meng Yang, Yongliang Zhang, Andrew O. Jackson, Dawei Li*, 2021, Hordeiviruses (Virgaviridae). In: Encyclopedia of Virology (Fourth Edition), Bamford D.H., Zuckerman M. (eds.) pp. 420-429. Academic Press: Oxford Xuan Zhang, Xueting Wang, Kai Xu, Zhihao Jiang, Kai Dong, Xialin Xie, He Zhang, Ning Yue, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, and Dawei Li*, 2021, The serine/threonine/tyrosine kinase STY46 defends against hordeivirus infection by phosphorylating γb protein, Plant Physiology, 186(1): 715-730. Xinxin Yang+, Zhiyan Wen+, Dingliang Zhang, Zhen Li, Dawei Li, Ugrappa Nagalakshmi, Savithramma P. Dinesh-Kumar*, Yongliang Zhang*, 2021, Proximity labeling: An emerging tool for probing in planta molecular interactions, Plant Communications, 2(2): 100137. Xueting Wang, Zhihao Jiang, Ning Yue, Xuejiao Jin, Xuan Zhang, Zhaolei Li, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, and Dawei Li*, 2021, Barley stripe mosaic virus γb protein disrupts chloroplast antioxidant defenses to optimize viral replication, The EMBO Journal, 40(16): e107660. Tingdong Li+, Jiacheng Hu+, Yu Sun, Boshu Li, Dingliang Zhang, Wenli Li, Jinxing Liu, Dawei Li, Caixia Gao, Yongliang Zhang*, Yanpeng Wang*, 2021, Highly efficient heritable genome editing in wheat using an RNA virus and bypassing tissue culture, Molecular Plant, 14(11): 1787-1798. Zhihao Jiang*, Meng Yang, Qing Cao, Yongliang Zhang, Dawei Li*, 2022, A powerful method for studying protein-protein interactions in plants: Co-immunoprecipitation (Co-IP) assay, in: Plant Virology: Methods and Protocols, Aiming Wang and Yi Li (eds), pp. 87-92. Methods in Molecular Biology, Vol. 2400. Humana, New York, NY. Meng Yang, Asigul Ismayil, Zhihao Jiang, Yan Wang, Xiyin Zheng, Yiguo Hong, Dawei Li*, Yule Liu*, 2022, A viral protein disrupts vacuolar acidification to facilitate virus infection in plants, The EMBO Journal, 41(2): e108713. Zongyu Gao, Dingliang Zhang, Xiaoling Wang, Xin Zhang, Zhiyan Wen, Qianshen Zhang, Dawei Li, Savithramma P. Dinesh-Kumar, Yongliang Zhang*, 2022, Coat proteins of necroviruses target 14-3-3a to subvert MAPKKKα-mediated antiviral immunity in plants, Nature Communications, 13(1): 716. Meng Yang, Yan Wang, Dawei Li, Yule Liu*, 2022, Plant virus infection disrupts vacuolar acidification and autophagic degradation for the effective infection, Autophagy, 18(3): 705-706. Zhaolei Li+, Xinxin Yang+, Wenli Li, Zhiyan Wen, Jiangning Duan, Zhihao Jiang, Dingliang Zhang, Xialin Xie, Xueting Wang, Fangfang Li, Dawei Li, Yongliang Zhang*, 2022, SAMDC3 enhances resistance to Barley stripe mosaic virus by promoting the ubiquitination and proteasomal degradation of viral γb protein, New Phytologist, 234(2): 618-633. Zhihao Jiang+*, Xuejiao Jin+, Meng Yang+, Qinglin Pi, Qing Cao, Zhenggang Li, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2022, Barley stripe mosaic virus γb protein targets thioredoxin h-type 1 to dampen salicylic acid-mediated defenses, Plant Physiology, [ https://doi.org/10.1093/plphys/kiac137 ] Ning Yue, Zhihao Jiang, Xuan Zhang, Zhenggang Li, Xueting Wang, Zhiyan Wen, Zongyu Gao, Qinglin Pi, Yongliang Zhang, Xian-Bing Wang, Chenggui Han, Jialin Yu, Dawei Li*, 2022, Palmitoylation of γb protein directs a dynamic switch between Barley stripe mosaic virus replication and movement, The EMBO Journal, [ https://doi.org/10.15252/embj.2021110060 ]
(* Corresponding author)
获奖情况
获国家教委科技进步一等奖（1997） 北京市科学技术二等奖（2002） 教育部二等奖（自然科学类）（2003） 河南省科学技术进步二等奖（2008）
承担项目及课题
专利情况
授权专利：甜菜黑色焦枯病毒作为外源基因的表达载体（ZL 02155378.5）
社会兼职
2014-2022 中国植物病理学会 理事
《植物病理学报》、《生物工程学报》 编委
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