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陈文峰
发布日期：2018-09-14 浏览次数：　　信息来源：生物学院

教师简介

姓名：陈文峰            
性别：男
系别：微生物学与免疫学
职称：副教授
学位：博士











陈文峰 博士，副教授。

科研方面，围绕根瘤菌与豆科植物共生固氮关系开展研究，涉及根瘤菌的多样性、系统发育、生物地理学、分子生态学、根瘤菌与豆科植物的分子识别与信号交流，根瘤菌的适应性进化和代谢调控对共生固氮效率的影响，高活性根瘤菌菌剂研制及农业生产上的应用示范等研究。教学方面，主要从事《微生物学》的理论课及实验课教学工作。


学习与工作经历

1992.9 ～ 1996.7 河南师范大学生物系 本科

1996.9 ～ 1999.6 云南大学微生物所 硕士

1999.9 ～ 2002.6 中国农业大学生物学院 博士

2002.10 ～2005.12 中国农业大学生物学院 讲师

2006.1至今中国农业大学生物学院 副教授 硕士生导师

 


研究方向

     共生固氮。重点解析影响根瘤菌与豆科植物共生固氮关系、共生固氮效率的内在和外在分子机制。


主讲课程

《微生物学》理论课和实验课。


联系方式

电话：62734009
邮件：chenwf at cau.edu.cn
通讯地址：


发表文章

科研相关论文(*为通讯作者)：

  1. 陈文峰,蒋凌月,徐丽华,姜成林.气质联用定量分析放线菌全细胞糖的研究.微生物学通报. 2000 , 27(6):416-420. 

  2. 陈文峰,陈文新.我国豆科植物根瘤菌资源多样性及应用基础研究.生物学通报.2003.38(7):1-4. 

  3. 陈文峰,陈文新.刺槐、黄檀、合欢根瘤菌的数值分类及16S rDNA-PCR RFLP研究.应用与环境生物学报. 2003. 41:53-58. 

  4. 陈文峰,刘杰,陈文新.木本豆科植物根瘤菌资源及多样性研究进展.科学技术与工程. 2004 , 4(11) , 954-959. 

  5. 陈文峰,李洪波. 3株紫花苜蓿根瘤菌sinR基因的克隆、序列测定及系统发育分析.应用与环境生物学报. 2008. 14(2),240-243. 

  6. 陈文峰. Sinorhizobium meliloti应译为草木樨中华根瘤菌. 中国科技术语. 2008. 10(3):62-63. 

  7. 刘晓云,陈文峰,陈文新.根瘤菌的系统发育及其分类研究进展.微生物学通报. 2003, 29:73-76. 

  8. 郑君芳,陈文峰,刘桂荣,隋新华,朱万孚,陈文新, R. N. Johnston , 刘树林.根瘤菌基因组结构的多样性及其系统发育的关系.微生物学通报.2003 , 30(4)：72-76.

  9. 陈文新,汪恩涛,陈文峰.根瘤菌-豆科植物共生多样性与地理环境的关系.中国农业科学. 2004. 37(1):81-86. 

  10. 陈文新,陈文峰.发挥生物固氮作用，减少化学氮肥用量.农业科技导报. 2004. 6(6):3-6. 

  11. 谷峻,陈文峰等.根瘤菌的群体感应研究进展.微生物学通报. 2004. 31(6):110-114. 

  12. 刘忠霞,陈文峰.教学用鞭毛染色的良好菌种－－灿烂类芽孢杆菌的鉴定.生物学通报. 2007. 42(6):48-50. 

  13. 13.陈世琼,陈文峰等. 16S rDNA PCR-RFLP法快速鉴定分离自浓缩苹果汁生产线的脂环酸芽孢杆菌.中国食品学报. 2006. 6(2):99-102. 

  14. 严雪瑞,陈文峰,陈文新,傅俊范,周如军,隋新华.辽宁营口地区锦鸡儿与树锦鸡儿根瘤菌遗传多样性的研究.沈阳农业大学学报, 2007,38(1): 44-48. 

  15. 严雪瑞,陈文峰,陈文新,傅俊范,薛彩云,隋新华.小叶锦鸡儿根瘤菌16S rDNA PCR-RFLP分析.华中农业大学学报，2007. 26(2):141-146. 

  16. 雷霞,陈文峰,隋新华,陈文新.根瘤菌多相分类研究进展.微生物学杂志. 2007. 27(6):77-80. 

  17. Tian Shen Tao*, Ying-yu Yue , Wen Xin Chen , Wen Feng Chen. Proposal of Nakamurella gen. nov. as a Substitute for the Bacterial Genus Microsphaera Yoshimi et al. 1996 , and Nakamurellaceae fam. nov. as a Substitute for the Illegitimate Bacterial Family Microsphaeraceae Rainey et al. 1997. International Journal of Systematic and Evolutionary Microbiology. 2004 , 54:999-1000. 

  18. Gui Xiang Peng , Zhi Yuan Tan , En Tao Wang , Barbara Reinhold-Hurek , Wen Feng Chen and Wen Xin Chen*. Identification of isolates from soybean nodules in Xinjiang Region as Sinorhizobium xinjiangense and genetic differentiation of S. xin, jiangense from Sinorhizobium fredii. International Journal of Systematic and Evolutionary Microbiology. 2002, 52:457–462.

  19. Xue Rui Yan, Wen Feng Chen*, Jun Fan Fu, Yang Li Lu, Cai Yun Xue, Xin Hua Sui, Ying Li, En Tao, Wang and Wen Xin Chen. Mesorhizobium spp. are the main microsymbionts of Caragana spp. grown in Liaoning Province of China. FEMS Microbiol Lett. 2007. 271:265-273.

  20. Wen Feng Chen*, Su Hua Guan, Chun Tian Zhao, Xue Rui Yan, Chao Xin Man, En Tao Wang & Wen Xin Chen. Different Mesorhizobium species associated with Caragana carried similar symbiotic genes and had common host range. FEMS Microbiol Lett. 2008. 283:203-209.

  21. Su Hua Guan, Wen Feng Chen*, En Tao Wang，Yang Li Lu，Xue Rui Yan, Xiao Xia Zhang and Wen Xin Chen. Mesorhizobium caraganae sp. nov., a novel rhizobial species nodulated with Caragana spp. in China. Int J Syst Evol Microbiol. 2008, 58: 2646 - 2653.

  22. Wang FQ, Wang ET, Liu J, Chen Q, Sui XH, Chen WF, Chen WX*. Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China. Int J Syst Evol Microbiol. 2007 Jun;57(Pt 6):1192-1199. 

  23. Gu CT, Wang ET, Sui XH, Chen WF, Chen WX. Persity and geographical distribution of rhizobia associated with Lespedeza spp. in temperate and subtropical regions of China. Arch Microbiol. 2007 Oct;188(4):355-365.

  24. Quan ZX*, Bae HS, Baek JH, Chen WF, Im WT, Lee ST. Rhizobium daejeonense sp. nov. isolated from a cyanide treatment bioreactor. Int J Syst Evol Microbiol. 2005 Nov;55(Pt 6):2543-2539. 

  25. Yong Fa Zhang, En Tao Wang, Chang Fu Tian, Feng Qin Wang, Li Li Han, Wen Feng Chen, and Wen Xin Chen*. Bradyrhizobium elkanii, B. yuanmingense and B. japonicum are the main rhizobia associated with Vigna unguiculata and Vigna radiata in the subtropical region of China. FEMS Microbiol Lett. 2008. 258:146-154.

  26. Ji Hong Li, En Tao Wang, Wen Feng Chen, Wen Xin Chen*. Genetic Persity and potential for promotion of plant growth detected in nodule endophytic bacteria of soybean grown in Heilongjiang province of China. Soil Biology & Biochemistry. 2008. 40:238-246.

  27. Man CX, Wang H, Chen WF, Sui XH, Wang ET, Chen WX* (2008) Diverse rhizobia associated with soybean grown in the subtropical and tropical regions of China. Plant Soil 310:77–87

  28. Y. L. Lu, W. F. Chen*, L. L. Han, E. T. Wang, X. X. Zhang, W. X. Chen and S. Z. Han. Mesorhizobium shangrilense sp. nov., isolated from root nodules of Caragana spp. growing in Yunnan Province of China. Int J Syst Evol Microbiol. 2009.

  29. Y. L. Lu, W. F. Chen, L. L. Han, E. T. Wang and W. X. Chen*. Rhizobium alkalisoli sp. nov., isolated from Caragana intermedia growing in saline-alkaline soils in the north of China. Int J Syst Evol Microbiol. 2009,

  30. Lin DX#, Chen WF#, Wang FQ, Hu D, Wang ET, Sui XH and Chen WX*. Rhizobium mesosinicum sp. nov., isolated from root nodules of three different legumes. Int J Syst Evol Microbiol. 2009, (# contribute equally to this article)

  31. Lu YL, Chen WF*, Wang ET, Guan SH, Yan XR, Chen WX.Genetic Persity and biogeography of rhizobia associated with Caragana species in three ecological regions of China. Syst Appl Microbiol. 2009 32: 351-361. (*Corresponding author). 

  32. Ren DW, Wang ET*, Chen WF, Sui XH, Zhang XX, Liu HC, Chen WX. Rhizobium herbae sp. nov. and Rhizobium giardinii-related bacteria, minor microsymbionts of various wild legumes in China. Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1912-20.

  33. Ren DW, Chen WF, Sui XH, Wang ET, Chen WX. Rhizobium vignae sp. nov., a symbiotic bacterium isolated from multiple legume species. Int J Syst Evol Microbiol. 2011 Mar;61(Pt 3):580-6.

  34. Li QQ, Wang ET, Zhang YZ, Zhang YM, Tian CF, Sui XH, Chen WF, Chen WX. Diversity and biogeography of rhizobia isolated from root nodules of Glycine max grown in Hebei Province, China. Microb Ecol. 2011 May;61(4):917-31.

  35. Li QQ, Wang ET, Chang YL, Zhang YZ, Zhang YM, Sui XH, Chen WF Chen WX*,. Ensifer sojae sp. nov., isolated from root nodules of Glycine max grown in saline-alkaline soils. Int J Syst Evol Microbiol. 2011 Aug;61(Pt 8):1981-8.

  36. Zhang YM, Li Y Jr, Chen WF*, Wang ET, Tian CF, Li QQ, Zhang YZ, Sui XH, Chen WX. Biodiversity and biogeography of rhizobia associated with soybean plants grown in the North China Plain. Appl Environ Microbiol. 2011 Sep;77(18):6331-42.

  37. Zhang YZ, Wang ET, Li M, Li QQ, Zhang YM, Zhang SJ, Jia XL, Zhang LH, Chen WF*, Chen WX. Effects of rhizobial inoculation, cropping systems and growth stages on endophytic bacterial community of soybean roots. Plant Soil, 2011, 347:147.

  38. Li M, Li Y, Chen WF*, Sui XH, Li Y Jr, Li Y, Wang ET, Chen WX. Genetic diversity, community structure and distribution of rhizobia in the root nodules of Caragana spp. from arid and semi-arid alkaline deserts, in the north of China. Syst Appl Microbiol. 2012 Jun;35(4):239-45.

  39. Zhang JJ, Liu TY, Chen WF*, Wang ET, Sui XH, Zhang XX, Li Y, Li Y, Chen WX. Mesorhizobium muleiense sp. nov., nodulating with Cicer arietinum L. Int J Syst Evol Microbiol. 2012 Nov;62(Pt 11):2737-42.

  40. Zhang JJ, Lou K, Jin X, Mao PH, Wang ET, Tian CF, Sui XH, Chen WF*. Distinctive Mesorhizobium populations associated with Cicer arietinum L. in alkaline soils of Xinjiang, China. Plant and Soil. 2012, Volume 353, Issue 1–2, pp 123–134.

  41. Zhao CT, Wang ET, Zhang YM, Chen WF, Sui XH, Chen WX*, Liu HC, Zhang XX. Mesorhizobium silamurunense sp. nov., isolated from root nodules of Astragalus species. Int J Syst Evol Microbiol. 2012 Sep;62(Pt 9):2180-6.

  42. Zhang YM, Li Y Jr, Chen WF*, Wang ET, Sui XH, Li QQ, Zhang YZ, Zhou YG, Chen WX. Bradyrhizobium huanghuaihaiense sp. nov., an effective symbiotic bacterium isolated from soybean (Glycine max L.) nodules. Int J Syst Evol Microbiol. 2012 Aug;62(Pt 8):1951-7.

  43. Zhang YZ, Chen WF*, Li M, Sui XH, Liu HC, Zhang XX, Chen WX. Bacillus endoradicis sp. nov., an endophytic bacterium isolated from soybean root. Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):359-63.

  44. Liu TY, Li Y Jr, Liu XX, Sui XH, Zhang XX, Wang ET, Chen WX, Chen WF, Puławska J. Rhizobium cauense sp. nov., isolated from root nodules of the herbaceous legume Kummerowia stipulacea grown in campus lawn soil. Syst Appl Microbiol. 2012 Oct;35(7):415-20.

  45. Zhang YM, Tian CF, Sui XH, Chen WF, Chen WX. Robust markers reflecting phylogeny and taxonomy of rhizobia. PLoS One. 2012;7(9):e44936.

  46. Li Y, Tian CF, Chen WF, Wang L, Sui XH, Chen WX. High-resolution transcriptomic analyses of Sinorhizobium sp. NGR234 bacteroids in determinate nodules of Vigna unguiculata and indeterminate nodules of Leucaena leucocephala. PLoS One. 2013 Aug 2;8(8):e70531.

  47. Wang YC, Wang F, Hou BC, Wang ET, Chen WF*, Sui XH, Chen WX, Li Y, Zhang YB. Proposal of Ensifer psoraleae sp. nov., Ensifer sesbaniae sp. nov., Ensifer morelense comb. nov. and Ensifer americanum comb. nov. Syst Appl Microbiol. 2013 Oct;36(7):467-73.

  48. Wang JY, Wang R, Zhang YM, Liu HC, Chen WF, Wang ET, Sui XH, Chen WX. Bradyrhizobium daqingense sp. nov., isolated from soybean nodules. Int J Syst Evol Microbiol. 2013 Feb;63(Pt 2):616-24.

  49. Ji ZJ, Wang FM, Wang SG, Yang SH, Guo R, Tang RY, Chen WX, Chen WF. [Symbiotic matching between soybean cultivar Luhuang No. 1 and different rhizobia].  Ying Yong Sheng Tai Xue Bao. 2014 Dec;25(12):3573-9. Chinese.

  50. Zhang JJ, Yu T, Lou K, Mao PH, Wang ET, Chen WF*, Chen WX. Genotypic alteration and competitive nodulation of Mesorhizobium muleiense against exotic chickpea rhizobia in alkaline soils. Syst Appl Microbiol. 2014 Oct;37(7):520-4.

  51. Mnasri B, Liu TY, Saidi S, Chen WF, Chen WX, Zhang XX, Mhamdi R. Rhizobium azibense sp. nov., a nitrogen fixing bacterium isolated from root-nodules of Phaseolus vulgaris. Int J Syst Evol Microbiol. 2014 May;64(Pt 5):1501-6.

  52. Jiao YS, Liu YH, Yan H, Wang ET, Tian CF, Chen WX, Guo BL, Chen WF*. Rhizobial Diversity and Nodulation Characteristics of the Extremely Promiscuous Legume Sophora flavescens. Mol Plant Microbe Interact. 2015 Dec;28(12):1338-52.

  53. Jiao YS, Yan H, Ji ZJ, Liu YH, Sui XH, Zhang XX, Wang ET, Chen WX, Chen WF*. Phyllobacterium sophorae sp. nov., a symbiotic bacterium isolated from root nodules of Sophora flavescens. Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):399-406.

  54. Jiao YS, Yan H, Ji ZJ, Liu YH, Sui XH, Wang ET, Guo BL, Chen WX, Chen WF. Rhizobium sophorae sp. nov. and Rhizobium sophoriradicis sp. nov., nitrogen-fixing rhizobial symbionts of the medicinal legume Sophora flavescens. Int J Syst Evol Microbiol. 2015 Feb;65(Pt 2):497-503.

  55. Yan H, Yan J, Sui XH, Wang ET, Chen WX, Zhang XX, Chen WF*. Ensifer glycinis sp. nov., a rhizobial species associated with species of the genus Glycine. Int J Syst Evol Microbiol. 2016 Sep;66(8):2910-6.

  56. Yan H, Ji ZJ, Jiao YS, Wang ET, Chen WF*, Guo BL, Chen WX. Genetic diversity and distribution of rhizobia associated with the medicinal legumes Astragalus spp. and Hedysarum polybotrys in agricultural soils. Syst Appl Microbiol. 2016 Mar;39(2):141-9.

  57. Chen WH, Yang SH, Li ZH, Zhang XX, Sui XH, Wang ET, Chen WX, Chen WF*. Ensifer shofinae sp. nov., a novel rhizobial species isolated from root nodules of soybean (Glycine max). Syst Appl Microbiol. 2017 Apr;40(3):144-149.

  58. Ji ZJ, Yan H, Cui QG, Wang ET, Chen WF, Chen WX. Competition between rhizobia under different environmental conditions affects the nodulation of a legume. Syst Appl Microbiol. 2017 Mar;40(2):114-119.

  59. Yan J, Yan H, Liu LX, Chen WF, Zhang XX, Verástegui-Valdés MM, Wang ET, Han XZ. Rhizobium hidalgonense sp. nov., a nodule endophytic bacterium of Phaseolus vulgaris in acid soil. Arch Microbiol. 2017 Jan;199(1):97-104.

  60. Yan H, Xie JB, Ji ZJ, Yuan N, Tian CF, Ji SK, Wu ZY, Zhong L, Chen WX, Du ZL, Wang ET, Chen WF*. Evolutionarily Conserved nodE, nodO, T1SS, and Hydrogenase System in Rhizobia of Astragalus membranaceus and Caragana intermedia. Front Microbiol. 2017 Nov 20;8:2282. doi: 10.3389/fmicb.2017.02282.

  61. Yan J, Li Y, Yan H, Chen WF, Zhang X, Wang ET, Han XZ, Xie ZH. Agrobacterium salinitolerans sp. nov., a saline-alkaline-tolerant bacterium isolated from root nodule of Sesbania cannabina. Int J Syst Evol Microbiol. 2017 Jun;67(6):1906-1911.

  62. Yan J, Li Y, Han XZ, Chen WF, Zou WX, Xie Z, Li M. Agrobacterium deltaense sp. nov., an endophytic bacteria isolated from nodule of Sesbania cannabina. Arch Microbiol. 2017 Sep;199(7):1003-1009.

  63. Zhang JJ*, Chen Y, Philippe de L, Singh, RP, Wang, ET, Chen WF. Mesorhizobium muleiense and Mesorhizobium gsp. nov. are symbionts of Cicer arietinum L. in alkaline soils of Gansu, Northwest China. Plant and Soil. 2017, Volume 410, Issue 1–2, pp 103–112.

  64. Yan J*, Chen WF, Han XZ, Wang ET, Zou WX, Zhang ZM. Genetic diversity of indigenous soybean-nodulating rhizobia in response to locally-based long term fertilization in a Mollisol of Northeast China. World Journal of Microbiology and Biotechnology. 2017, 33:6.

  65. Liu YH, Wang ET, Jiao YS, Tian CF, Wang L, Wang ZJ, Guan JJ, Singh RP, Chen WX, Chen WF*.Symbiotic characteristics of Bradyrhizobium diazoefficiens USDA 110 mutants associated with shrubby sophora (Sophora flavescens) and soybean (Glycine max). Microbiol Res. 2018 Sep;214:19-27.

  66. Yang SH, Chen WH, Wang ET, Chen WF*, Yan J, Han XZ, Tian CF, Sui XH, Singh RP, Jiang GM, Chen WX. Rhizobial biogeography and inoculation application to soybean in four regions across China. J Appl Microbiol. 2018 Sep;125(3):853-866.

  67. Hu Y, Jiao J, Liu LX, Sun YW, Chen WF, Sui XH, Chen WX, Tian CF*. Evidence for Phosphate Starvation of Rhizobia without Terminal Differentiation in Legume Nodules. Mol Plant Microbe Interact. 2018 Aug 20:MPMI02180031R. doi: 10.1094/MPMI-02-18-0031-R.

  68. Liu YH, Jiao YS, Liu LX, Wang D, Tian CF, Wang ET, Wang L, Chen WX, Wu SY, Guo BL, Guan ZG, Poinsot V, Chen WF*. Nonspecific Symbiosis Between Sophora flavescens and Different Rhizobia. Mol Plant Microbe Interact. 2018 Feb;31(2):224-232.

  69. Zhang JJ*, Guo C, Chen WF, de Lajudie P, Zhang ZY, Shang YM, Wang ET. Mesorhizobium wenxiniae sp. nov., isolated from chickpea (Cicer arietinum L.) in China. 20 April 2018, International Journal of Systematic and Evolutionary Microbiology 68: 1930-193.

  70. Zhang JJ*, Guo C, Chen WF, Shang YM, Lajudie Philippe de, Yang X, Mao PH, Zheng JQ, Wang ET. Dynamic succession of chickpea rhizobia over years and sampling sites in Xinjiang, China. Plant and Soil, 2018, 425 (1-2), p. 241-251.


书籍编撰：

《原核生物系统学》副主编，化学工业出版社。2007. （主编：陶天申，东秀珠，杨瑞馥）

《中国根瘤菌》副主编，科学出版社，2011. 主编：陈文新，汪恩涛；副主编：陈文峰、田长富。


教学相关论文：
　　陈文峰. 在教学实践中提高青年微生物学教师的教学能力. 教育教学改革实践与探索. 2004. 
　　陈文峰, 王颖, 袁红莉等. 高等农业院校农业微生物学立体开放实验和系统建设.微生物教学与科研新进展. 2005.
　　关国华， 陈文峰， 李颖等. 新版的微生物生理学;. 微生物教学与科研新进展. 2005.
　　王颖, 袁红莉, 陈文峰等.农业微生物学课程的量体裁衣式教学的探讨. 微生物教学与科研新进展. 2005.
　　王颖, 李颖, 陈文峰.微生物生物学教学改革几点尝试. 教育教学改革实践与探索， 2005，2：92-94.
　　陈文峰, 袁红莉, 王颖, 李宝珍. 农业微生物学开放实验课选课系统的设计与实现. 教育教学改革实践与探索. 2006.
　　陈文峰, 关国华, 李颖, 袁红莉. 多角度、多层次提升本科生的教学与管理水平－－上海交通大学、复旦大学、浙江大学教学考察总结与体会. 第11届微生物学教学与科研研讨会论文集. 2007. 中国新乡.
　　陈文峰, 袁红莉, 李宝珍, 王颖. 全方位推进农业微生物学精品课程建设。教育教学改革实践与探索（迎评专辑），中国农大出版社，2007，9，p45-47.
　　刘忠霞, 陈文峰*. 微生物实验教学用鞭毛染色的良好菌种－－灿烂类芽孢杆菌的鉴定. 生物学通报. 2007. 42(6):48-50. （*通讯作者）
　　陈文峰. 充分发挥多媒体课件的优势提高课堂教学效果. 教育教学改革实践与探索. 2009年4月出版发行. 136-138.
　　袁红莉, 李宝珍, 陈文峰等其他11名作者. 精品课程《农业微生物学》阶段性建设成果. 教育教学改革实践与探索. 2009年4月出版发行. 95-97.


获奖情况

2008年度高等学校科学研究优秀成果奖（科学技术）：17属豆科植物根瘤菌遗传多样性研究及7个根瘤菌新种的发表，获奖人：陈文新,陈文峰,隋新华,李颖,韦革宏,王风芹,韩素贞,刘杰,刘晓云,严雪瑞。


承担项目及课题

       在研主持国家自然科学基金面上项目：不同根瘤菌与苦参的混杂性共生机制研究，2018-2021.


专利情况

1、菌剂双联袋(豆乐宝)，专利号ZL2016302130298。

2、一株根瘤菌及其在柠条育苗中的应用的制作方法，专利号201310292237.7。


社会兼职

       中国微生物学会会员，农业微生物学会会员。
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