Yi Yang, Jinshui Yang, Baozhen Li, Entao Wang, Hongli Yuan. An esterase from Penicillium decumbens P6 involved in lignite depolymerization. Fuel. DOI: org/10.1016/j.fuel​
发布日期:2019-11-11 浏览次数:  信息来源:生物学院

An esterase from Penicillium decumbens P6 involved in lignite depolymerization

Yi Yang, Jinshui Yang, Baozhen Li, Entao Wang, Hongli Yuan


Fuel

DOI: org/10.1016/j.fuel


ABSTRACT

In this study, lignite was degraded using a purified esterase from Penicillium decumbens P6 for the first time. The esterase was purified using ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The recovery and purification yield of the enzyme were 15% and 83 folds, respectively. The molecular weight of the purified enzyme was about 45kDa. Both crude and purified esterases were studied for lignite depolymerization. The tendency of depolymerization by crude enzyme was consistent with the enzyme secreted in the medium. Along with the increased purified esterase concentration from 8 to 50mg/ml, A450 value increased from 0.38to 2.08. Thecontribution ofesterase to the depolymerization was about 40%in thecrude supernatant. Compared with aHA (crude lignite humic acid), bHA (esterase degraded lignite humic acid) has a lower percentage of aromatic carbon and ester groups, but a higher percentage of aliphatic carbon. bHA can promote the growth of asparagus lettuce. The results demonstrated that lignite was depolymerized by the purified esterase and evidenced the potential of esterase application in conversion of lignite into compounds with high bioactivities.

 

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