|Keke Xia, Bo Wang, Jiewei Zhang, Yuan Li, Hailian Yang & Dongtao Ren. Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance. Plant, Cell and Environment. DOI: 10.1111/pce.12918|
Arabidopsis phosphoinositide-specific phospholipase C 4 negatively regulates seedling salt tolerance
Keke Xia, Bo Wang, Jiewei Zhang, Yuan Li, Hailian Yang & Dongtao Ren
Plant, Cell and Environment
Previous physiological and pharmacological studies have suggested that the activity of phosphoinositide-speciﬁc phospholipase C (PI-PLC) plays an important role in regulating plant salt stress responses by altering the intracellular Ca2+ concentration.However,theindividualmembersofplant PLCs involved in this process need to be identiﬁed. Here, the function of AtPLC4 in the salt stress response of Arabidopsis seedlings was analysed. plc4 mutant seedlings showed hyposensitivity to salt stress compared with Col-0 wild-type seedlings, and the salt hyposensitive phenotype could be complemented by the expression of native promotercontrolled AtPLC4. Transgenic seedlings with AtPLC4 overexpression (AtPLC4 OE) exhibited a salt-hypersensitive phenotype, while transgenic seedlings with its inactive mutant expression (AtPLC4m OE) did not exhibit this phenotype. UsingaequorinasaCa2+indicatorinplc4mutantandAtPLC4 OE seedlings, AtPLC4 was shown to positively regulate the salt-induced Ca2+ increase. The salt-hypersensitive phenotype of AtPLC4 OE seedlings was partially rescued by EGTA. An analysisofsalt-responsivegenesrevealedthatthetranscription of RD29B, MYB15 and ZAT10 was inversely regulated in plc4 mutant and AtPLC4 OE seedlings. Our ﬁndings suggest that AtPLC4 negatively regulates the salt tolerance of Arabidopsisseedlings,andCa2+ maybeinvolvedinregulating this process.