|Kuo Zhang, Yuying Zhang, Lijie Gu, Tongtong Wang, Ning Li1 & Qingyong Meng. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy.NATURE COMMUNICATIONS.DOI: 10.1038/s41467-018-07|
Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy
Kuo Zhang, Yuying Zhang, Lijie Gu, Miaomiao Lan, Chuncheng Liu, Meng Wang, Yang Su, Mengxu Ge, Tongtong Wang, Yingying Yu, Chang Liu, Lei Li, Qiuyan Li, Yaofeng Zhao, Zhengquan Yu, Fudi Wang, Ning Li1 & Qingyong Meng
Satellite cells are crucial for skeletal muscle regeneration, but the molecular mechanisms regulating satellite cells are not entirely understood. Here, we show that the immunoglobulin superfamily containing leucine-rich repeat (Islr), a newly identiﬁed marker for mesenchymal stem cells, stabilizes canonical Wnt signaling and promote skeletal muscle regeneration. Loss of Islr delays skeletal muscle regeneration in adult mice. In the absence of Islr, myoblasts fail to develop into mature myotubes due to defective differentiation. Islr interacts with Dishevelled-2 (Dvl2) to activate canonical Wnt signaling, consequently regulating the myogenic factor myogenin (MyoG). Furthermore, Islr stabilizes Dvl2 by reducing the level of LC3-labeled Dvl2 and preventing cells from undergoing autophagy. Together, our ﬁndings identify Islr as an important regulator for skeletal muscle regeneration.