Lei Zhu, Liang-Cui Chu, Yan Liang, Xue-Qin Zhang, Li-Qun Chen and De Ye. The Arabidopsis CrRLK1L protein kinases BUPS1 and BUPS2are required for normal growth of pollen tubes in the pistil. The plant journal. DOI: 10.1111/tpj.13963
发布日期:2019-11-12 浏览次数:  信息来源:生物学院

The Arabidopsis CrRLK1L protein kinases BUPS1 and BUPS2are required for normal growth of pollen tubes in the pistil

Lei Zhu, Liang-Cui Chu, Yan Liang, Xue-Qin Zhang, Li-Qun Chen and De Ye

 

The plant journal

DOI: 10.1111/tpj.13963

 

ABSTRACT

In flowering plants, the interaction of pollen tubes with female tissues is important for the accomplishment of double fertilization. Little information is known about the mechanisms that underlie signalling between pollen tubes and female tissues. In this study, two Arabidopsis pollen tube-expressed CrRLK1L proteinkinases, Buddha’s Paper Seal 1 (BUPS1) and BUPS2, were identified as being required for normal tip growth of pollen tubes in the pistil. They are expressed prolifically in pollen and pollen tubes and are localized on the plasma membrane of the pollen tube tip region. Mutations inBUPS1drastically reduced seed set. Most of the bups1mutant pollen tubes growing in the pistil exhibited a swollen pollen tube tip, leading to failure of fertilization. The bups2pollen tubes had a slightly abnormal morphology but could still accomplish double fertilization. The bups1 bups2double mutant exhibited a slightly enhanced phenotype compared to the single bups1mutants. The BUPS1 proteins could form homomers and heteromers with BUPS2, whereas BUPS2 could only form heteromers with BUPS1. The BUPS proteins could interact with the Arabidopsis pollen-expressed RopGEFs in the yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. The results indicated that the BUPSs may mediate normal polar growth of pollen tubes in the pistil.


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