Yi Yang, Jinshui Yang, Jiawen Liu, Ruonan Wang, Liang Liu, Fengqin Wang, Hongli Yuan. The composition of accessory enzymes of Penicillium chrysogenum P33revealed by secretome and synergistic effects with commercial cellulase onlignocellulose hydrolysis
发布日期:2019-11-12 浏览次数:  信息来源:生物学院

The composition of accessory enzymes of Penicillium chrysogenum P33revealed by secretome and synergistic effects with commercial cellulase onlignocellulose hydrolysis

Yi Yang, Jinshui Yang, Jiawen Liu, Ruonan Wang, Liang Liu, Fengqin Wang, Hongli Yuan

 

Bioresource Technology

DOI: 10.1016/j.biortech.2018.02.028

 

ABSTRACT

Herein, we report the secretome of Penicillium chrysogenum P33 under induction of lignocellulose for the first time. A total of 356 proteins were identified, including complete cellulases and numerous hemicellulases. Supplementing a commercial cellulase with increasing dosage of P33 enzyme cocktail from 1 to 5 mg/g substrate increased the release of reducing sugars from delignified corn stover by 21.4% to 106.8%. When 50% cellulose was replaced by P33 enzyme cocktail, release of reducing sugars was 78.6% higher than with cellulase alone. Meanwhile, glucan and xylan conversion was increased by 37% and 106%, respectively. P33 enzyme cocktail also enhanced commercial cellulase hydrolysis against four different delignified lignocellulosic biomass. These findings demonstrate that mixing appropriate amount of P33 cocktail with cellulase improves polysaccharide hydrolysis, suggesting P33 enzymes have great potential for industrial applications.

 


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