Ziyan Liu,Shuhua Yang etal.Plasma Membrane CRPK1 -Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import
to Fine-Tune CBF Signaling during Cold Response.Molecular Cell.DOI:10.1016/j.molcel.2017.02.016
发布日期:2019-11-08 浏览次数:  信息来源:生物学院

Plasma Membrane CRPK1 -Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response

Ziyan Liu,Yuxin Jia, Yanglin Ding, Yiting Shi,Zhen Li, Yan Guo, Zhizhong Gong, and Shuhua Yang

Molecular Cell.

DOI:10.1016/j.molcel.2017.02.016  42,s170586,杨淑华.pdf


Abstract

In plant clls, changes in fuidity of the plasma membrane may serve as the primary sensor of cold stress;however, the precise mechanism and how the cell transduces and fine-tunes cold signals remain elusive. Here we show that the cold-activated plasma membrane protein cold-responsive protein kinase 1 (CRPK1) phosphorylates 14-3-3 proteins.The phosphorylated 14-3-3 proteins shuttle from the cytosol to the nucleus, where they interact with and destabilize the key cold-responsive C-repeatbinding factor (CBF)proteins. Consistent with this, the crpk1 and 14-3-3kλ mutants show enhanced freezing tolerance, and transgenic plants overexpressing 14-3-3λ show reduced freezing tolerance. Further study shows that CRPK1 is essential for the nuclear translocation of 14-3-3 proteins and for 14-3-3 function in freezing tolerance. Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF sabilit, which ensure a faithfully adjusted response to cold stress of plants.

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