|Ziyan Liu,Shuhua Yang etal.Plasma Membrane CRPK1 -Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import|
to Fine-Tune CBF Signaling during Cold Response.Molecular Cell.DOI:10.1016/j.molcel.2017.02.016
Plasma Membrane CRPK1 -Mediated Phosphorylation of 14-3-3 Proteins Induces Their Nuclear Import to Fine-Tune CBF Signaling during Cold Response
Ziyan Liu,Yuxin Jia, Yanglin Ding, Yiting Shi,Zhen Li, Yan Guo, Zhizhong Gong, and Shuhua Yang
In plant clls, changes in fuidity of the plasma membrane may serve as the primary sensor of cold stress;however, the precise mechanism and how the cell transduces and fine-tunes cold signals remain elusive. Here we show that the cold-activated plasma membrane protein cold-responsive protein kinase 1 (CRPK1) phosphorylates 14-3-3 proteins.The phosphorylated 14-3-3 proteins shuttle from the cytosol to the nucleus, where they interact with and destabilize the key cold-responsive C-repeatbinding factor (CBF)proteins. Consistent with this, the crpk1 and 14-3-3kλ mutants show enhanced freezing tolerance, and transgenic plants overexpressing 14-3-3λ show reduced freezing tolerance. Further study shows that CRPK1 is essential for the nuclear translocation of 14-3-3 proteins and for 14-3-3 function in freezing tolerance. Thus, our study reveals that the CRPK1-14-3-3 module transduces the cold signal from the plasma membrane to the nucleus to modulate CBF sabilit, which ensure a faithfully adjusted response to cold stress of plants.