Yang Su,Lei Xiong,Kun Wang,Ting He,Jianyun Shi,Yongli Song,Yaofeng Zhao,Ning Li,Zhengquan Yu,Qingyong Meng.Fate Decision of Satellite Cell Differentiation and Self-Renewal by miR-31-IL34 Axis.DOI: 10.1038/s41418-019-0390-x
发布日期:2020-04-28 浏览次数:  信息来源:生物学院

Fate Decision of Satellite Cell Differentiation and Self-Renewal by miR-31-IL34 Axis

Yang Su,Yingying Yu,Chuncheng Liu,Yuying Zhang,Chang Liu,Mengxu G,Lei Li,Miaomiao Lan,Tongtong Wang,Min Li,Fan Liu,Lei Xiong,Kun Wang,Ting He,Jianyun Shi,Yongli Song,Yaofeng Zhao,Ning Li,Zhengquan Yu,Qingyong Meng


Cell Death And Differentiation

DOI: 10.1038/s41418-019-0390-x



Abstract

Quiescent satellite cells (SCs) that are activated to produce numerous myoblasts underpin the complete healing of damaged skeletal muscle. How cell-autonomous regulatory mechanisms modulate the balance among cells committed to differentiation and those committed to self-renewal to maintain the stem cell pool remains poorly explored. Here, we show that miR-31 inactivation compromises muscle regeneration in adult mice by impairing the expansion of myoblasts. miR-31 is pivotal for SC proliferation, and its deletion promotes asymmetric cell fate segregation of proliferating cells, resulting in enhanced myogenic commitment and re-entry into quiescence. Further analysis revealed that miR-31 posttranscriptionally suppresses interleukin 34 (IL34) mRNA, the protein product of which activates JAK-STAT3 signaling required for myogenic progression. IL34 inhibition rescues the regenerative deficiency of miR-31 knockout mice. Our results provide evidence that targeting miR-31 or IL34 activities in SCs could be used to counteract the functional exhaustion of SCs in pathological conditions.


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