姜伟
发布日期:2018-09-18 浏览次数:3560  信息来源:生物学院

教师简介

姓名:姜伟            
性别:
系别:微生物学与免疫学
职称:教授
学位:博士











姜伟 博士,教授,博士研究生导师。1993年在武汉大学获得理学硕士学位,2003年在中国农业大学获得理学博士学位。2005-2006年日本国立食品研究所访问学者。近年来在《Critical Review in Biotechnology》、《Environmental Microbiology》、《Applied Microbiology and Biotechnology》、《Frontiers in Microbiology》、《Microbial Cell Factories》、《Research in Microbiology》、《Biotechnology for Biofuels》、《Bioresource Technology》、《Applied and Environmental Microbiology》、《Journal of Bacteriology》、《Scientific Report》等国际杂志上以通讯作者或合作者发表研究论文三十余篇。


学习与工作经历

1984/9 -1987/6,鄂西大学学习

1990/9 -1993/6,武汉大学,理学硕士学位

1999/9 -2003/3,中国农业大学,理学博士学位

1987/7 - 1990/9,湖北民族学院,生命科学院,助教

1993/7 - 1999/8, 湖北民族学院,生命科学院,讲师

2003/4 - 2005/4,中国农业大学,生物学院,讲师、副教授,硕士研究生导师

2005/4 - 2006/4,日本国立食品研究所 访问学者

2006/4 - 2022/12,中国农业大学,生物学院,副教授,2011年博士研究生导师

2023/1 - 至今,中国农业大学,生物学院,教授


研究方向

现主要从事趋磁细菌磁小体(纳米磁性颗粒)形成的分子机理、发酵制备及其在肿瘤靶向治疗中的应用研究;生物固氮中固氮酶还原底物和质子分子机理的研究;生物质(农作物秸秆)合成与降解相关酶系(纤维素酶、木聚糖酶、几丁质酶、甘露聚糖酶、半乳糖苷酶等)的表达、发酵及其应用研究;小分子多肽药物的表达、发酵与纯化研究。指导已毕业博士研究生4人,硕士研究生4人,在读博士研究生9人。 

主讲课程

《发酵工程》、《发酵工程实验》


联系方式

电话:010-62731440
邮件:jiangwei01 at cau.edu.cn
通讯地址:北京市海淀区圆明园西路2号中国农业大学西校区生命科学研究中心3036

 

发表文章(*通讯作者,#共同第一作者)

  1. Abdul Basit, Liu Junquan, Kashif Rahim, Jiang Wei*, Lou Huiqiang*, 2018, Thermophilic xylanases: from bench to bottle. Critical Review in Biotechnology, 38(7), 989–1002.

  2. Liu Junquan, Abdul Basit, Miao Ting, Zheng Fengzhen, Jiang Wei*, Cao Yunhe*, 2018, Secretory expression of β-mannanase in Saccharomyces cerevisiae and its high efficiency for hydrolysis of mannans to mannooligo- saccharides. Applied Microbiology and Biotechnology, https://doi.org/10. 1007/s00253-018-9355-0.

  3. Abdul Basit, Liu Junquan, Miao Ting, Zheng Fengzhen, Lou Huiqiang*, Jiang Wei*, 2018, Characterization of two endo-b-1, 4-xylanases from Myceliophthora thermophila and their saccharification efficiencies, synergistic with commercial cellulose. Frontiers in Microbiology, doi:10.3389/fmicb.2018. 00233.

  4. Wang Xi, Wang Jigui, Geng Yuanyuan, Wang Jiaojiao, Zhang Xiaomei, Yang Shuangshuang, Jiang Wei, Liu Weiquan. 2018, An enhanced anti-tumor effect of apoptin-cecropin B on human hepatoma cells by using bacterial magnetic particle gene delivery system. Biochemical and Biophysical Research Communications 496(2): 719- 725.

  5. Dai Qinglei, Long Ruimin, Wang Shibin, Ranjith Kumar Kankala, Wang Jiaojiao, Jiang Wei and Liu Yuangang*, 2017, Bacterial magnetosomes as an efficient gene delivery platform for cancer theranostics. Microbial Cell Factories, DOI 10.1186/s12934-017 -0830-6.

  6. Kadarkarai M, Jiang Wei, Mohamad SH, Marcello N, Manickam P, Christina MS, Devakumar D, Balamurugan C, et al., 2017, Magnetic nanoparticles are highly toxic to chloroquine-resistant Plasmodium falciparum, dengue virus (DEN-2), and their mosquito vectors. Parasitology Research 116: 495-502.

  7. Zhang Yunpeng, Wen Tong, Guo Fangfang, Geng Yuanyuan, Liu Junquan, Peng Tao, Guan Guohua, Tian Jiesheng, Li Ying, Li Jilun, Ju Jing *, Jiang Wei*, 2017, The disruption of an OxyR-like protein impairs intracellular magnetite biomineralization in Magnetospirillum gryphiswaldense MSR-1. Frontiers in Microbiology 8: 208.

  8. Wen Tong, Guo Fangfang, Zhang Yunpeng, Tian Jiesheng, Li Ying, Li Jilun, Jiang Wei*, 2016, A novel role for Crp in controlling magnetosome biosynthesis in Magnetospirillum gryphiswaldense MSR-1, Scientific Report, 6:21156 | DOI: 10.1038/srep21156.

  9. Zhang Chan, Meng Xia, Li Ningxiao, Wang Wei, Sun Yuan, Jiang Wei, Guan Guohua , Li Ying, 2013, Two bifunctional enzymes with ferric reduction ability play complementary roles during magnetosome synthesis in Magnetospirillum gryphiswaldense MSR-1. Journal of Bacteriology 195, 876-885.

  10. Wang Xu, Wang Qing, Zhang Weijia, Wang Yinjia, Li Li, Wen Tong, Zhang Tongwei, Zhang Yang, Xu Jun, Hu Junying, Li Shuqi, Liu Lingzi, Liu Jinxin, Jiang Wei, Tian Jiesheng, Li Ying, Dirk Schüler, Wang Lei, Li Jilun, 2014, Complete Genome Sequence of Magnetospirillum gryphiswaldense MSR-1. Genome A 2, 2 e00171-14.

  11. Xu Jun, Hu Junying, Liu Lingzi, Li Li, WangXu, Zhang Huiyuan, Jiang Wei, Tian Jiesheng, Li Ying and Li Jilun, 2014, Surface expression of protein A on magnetosomes and capture of pathogenic bacteria by magnetosome/antibody complexes, Frontiers in Microbiology 5, 136.

  12. Guo Qingjuan, Peng Tao, Chang Tianju, Zhang Gang, Jiang Wei, Li Ying, Li Jilun, 2014, Mutagenesis at α-423Ile of MoFe protein reduces the catalytic activity of nitrogenase in Klebsiella oxytoca, Chinese Science Bulletin, 59(9): 849-856.

  13. Huang Jinjin, Ji Xia, Yang Zhen, Guan Feifei, Cui Di, Guan Guohua, Jiang Wei and Li Ying2014, Improved production of a recombinant Rhizomucor miehei lipase expressed in Pichia pastoris and its application for conversion of microalgae oil to biodiesel. Biotechnology for Biofuels, 7, 111.

  14. Huang Jinjin, Ji Xia, Jiang Wei, Li Ying, Li Jilun, 2015, Biodiesel production from microalgae oil catalyzed by a recombinant lipase. Bioresource Technology 180, 47-53.

  15. Guo FangFang, YangWei, Jiang Wei*, Geng Shuang, Peng Tao, and Li JilunMagnetosomes eliminate intracellular reactive oxygen species in Magnetospirillum gryphiswaldense MSR-1Environmental Microbiology, 2012, 14(7), 1722–1729.

  16. Guo FangFang, Liu Yang, Cheng Yan-Ping, Tang Tao, Jiang Wei*, Li Ying, Li Jilun, 2011, A novel rapid and continuous procedure for large-scale purification of magnetosomes from Magnetospirillum gryphiswaldense, Applied Microbiology and Biotechnology, 9041277-1283.

  17. Zhangyang, Zhang Xiaojuan, Jiang Wei, Li Ying and Li Jilun, 2011, Semicontinuous culture of Magnetospirillum gryphiswaldense MSR-1cells in an autofermentor by nutrient-balanced and isosmotic feeding strategies. Applied and Environmental Microbiology, 77, 5851-5856. 

  18. Yang Wei, Li Rui-Guo, Peng Tao, Zhang Yang, Jiang Wei*, Li Ying, Li Jilun, 2010, mamO and mamE genes are essential for magnetosome crystal biomineralization in Magnetospirillum gryphiswaldense MSR-1, Research in Microbiology 161, 701-705.

  19. Liu Yang, Li Rui-Guo, Guo Fang-Fang, Jiang Wei*, Li Ying, Li Jilun, 2010, Large-scale production of magnetosomes by chemostat culture of Magnetospirillum gryphiswaldense at high cell density. Microbial Cell Factories 9: 99.

  20. Ban Jia, Jiang Wei, Li Ying, Zhang Yaoping, Li Jilun, 2010, Functional analysis of hydrogenases and their effects on cell growth and magnetosome synthesis in Magnetospirillum gryphiswaldense. Chinese Science Bulletin, 55: 140-147.

  21. Ding Yao, Li Jinhua, Liu Jiangning, Yang Jing, Jiang Wei, Tian Jiesheng, Li Ying, Pan Yongxin, Li Jilun, 2010, Deletion of the ftsZ-like gene results in the production of superparamagnetic magnetite magnetosomes in Magnetospirillum gryphiswaldense. Journal of Bacteriology 192: 1097-1105.

  22. Chen Jifeng, Li Ying, Wang Zenfang, Li Jilun, Jiang Wei, Li Saoha. 2009, High-sensitivity detection of fruit tree viruses using bacterial magnetic particles. Journal of Integrative Plant Biology 51(4):409-13.

  23. Li Feng , Li Ying, Jiang Wei,Wang Zhenfang , Li Jilun, 2009. Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM21 of  Magnetospirillum gryphiswaldense MSR-1 , Chinese Science Bulletin, 54 (9): 1522-1528. 

  24. Sun Jian-Bo, Zhao Feng, Tang Tao, Jiang Wei, Tian Jie-sheng, Li Ying, Li Ji-Lun, 2008, High-yield growth and magnetosome formation by Magnetospirillum gryphiswaldense MSR-1 in an oxygen-controlled fermentor supplied solely with air, Applied Microbiology and Biotechnology, 79: 389-397.

  25. Liu Jiangning, Ding Yao, Jiang Wei, Tian Jiesheng, Li Ying, Li Jilun, 2008,  A mutation upstream of an ATPase gene significantly increases magnetosome production in Magnetospirillum gryphiswaldense. Applied Microbiology and Biotechnology, 81:551–558. 

  26. Rong Chengbo, Huang Yijun, Zhang Weijia, Jiang Wei, Li Ying, Li Jilun, 2008, Ferrous iron transport protein B gene ( feoB1) plays an accessory role in magnetosome formation in Magnetospirillum gryphiswaldense strain MSR-1, Research in Microbiology, 159 530-536.

  27. Sun Jian-Bo, Duan Jin-Hong, Dai Shun-Ling, Ren Jun, Guo Lin, Jiang Wei, Li Ying, 2008, Preparation and anti-tumor efficiency evaluation of doxorubicin-loaded bacterial magnetosomes: magnetic nanoparticles as drug carriers isolated from Magnetospirillum gryphiswaldense, Biotechnology and Bioengeering. 101: 1313–1320.

  28. Li xiang, Jiang Wei, Sun Jianbo, Wang Guili, Guan Feng, Li Ying, 2007, Purified and sterilized magnetosomes from Magnetospirillum gryphiswaldense MSR-1 were not toxic to mouse fibroblasts in vitro, Letters in Applied Microbiology 45 (1)75-81.

  29. Meng xia, Jiang Wei, Yang Lei, Li Ying, 2007, A novel ferric reductase purified from Magnetospirillum gryphiswaldense MSR-1. Current Microbiology. 55 (1): 71-75.

  30. Huang Yijun, Zhang Weijia, Jiang Wei, Rong Chengbo, Li Ying, 2007, Disruption of the fur gene inhibits magnetosome formation in Magnetospirillum gryphiswaldense . Biochemistry (Moscow) 7211):1247-1253.

  31. Li Xiang, Wang Bin, Jin Huali, Jiang Wei, Tian Jiesheng, Guan Feng, Li Ying,  2007, Bacterial magnetic particles (BMPs)-PEI as a novel and efficient non-viral gene delivery system, Journal of Gene Medicine, 9: 679-690.

  32. Guan Feng, Zhao Dehua, Pan Miao, Jiang Wei, Li Jilun, 2007, Analysis of active sites for N2 and H+ reduction on FeMo-cofactor of nitrogenase. Chinese Science Bulletin, 52(15):2088-2094.

  33. Lifeng, Li Ying and Jiang Wei et al., 2005, Cloning and functional analysis of the sequences flanking mini-Tn5 in the magnetosomes deleted mutant NM4 of Magnetospirillum gryphiswaldense MSR-1. Science in China Series C-Life Sciences, Vol.48, No.6, 574-584.

  34. Jiang Wei , Sun Jian-bo  and Li Ying et al, 2005, The effect of conditions on the growth of Magnetospirillum gryphiswaldense , China Journal of Modern Medicine, 1523):3521-3526.

  35. JiangWei, LiYing and Li Jilun, 2005, Activity of dinitrogenase reductase is regulated by ADP-ribosylation in Magnetospirillum gryphiswaldense. Biological Nitrogen Fixation, Sustainable Agriculture and the Environment, p97.

  36. JiangWei, Zhao Dehua and LiYing et al., 2002, Submerged culture of Magnetospirillum gryphiswaldense under N2-fixing condition and regulation of activity of nitrogen fixation, Chinese Science Bulletin, 47(24): 2095-2099.

  37. 王姣姣,耿圆圆,张家耀*,姜伟,肝细胞癌循环肿瘤细胞标志物及检测方法研究进展,生物技术进展,2015,(5)(2):113-119。

  38. 郭芳芳,杨薇,姜伟*,细菌纳米磁小体的合成机制及应用,生物技术进展,2012,1: 16-22.

  39. 张云鹏,温彤,姜伟*,大肠杆菌和酵母表达系统的研究进展,生物技术进展,2014, 4(6):89-393。

  40. 陈彦平,郭芳芳,姜伟,李颖,2011, 趋磁细菌纳米磁小体的特征及标准化检测体系,东南大学学报, 30(1):47-51。

  41. 刘聪,李颖,李季伦,姜伟,田杰生,2010,抗氧化酶过表达对趋磁螺菌MSR-1耐氧性的影响,《生物技术通报》 年10期,216-221。

  42. 孙建波,姜伟,李颖,张阳德,李季伦, 2007, 细菌纳米磁小体有望作为靶向药物载体, 微生物学通报,34(1):165-168。

  43. 姜伟,田杰生,李颖,李季伦,2007,趋磁细菌的特点及其纳米磁小体合成条件,中国农业科技导报,9(3):24-31。

  44. 关锋,赵德华,潘淼,姜伟,李季伦,2007, N2 和H+在固氮酶活性中心金属原子簇中还原位点的分析,科学通报,52:1141-1146。

  45. 陈继峰,李颖,姜伟等,2006,结合条件对羊抗兔抗体连接到细菌磁小体的影响,农业生物技术学报, 14(3):423-428。

  46. 姜伟,付刚,李颖等,2004,细菌内磁纳米粒研究,中国医学工程,11(6):59-62。

  47. 付刚,李颖,姜伟等,2004,磁螺菌磁小体形成的电镜观察及其纯化,中国现代医学杂志,14(5):45~49。

  48. 李峰,李颖,姜伟等,2004, 趋磁螺菌遗传操作体系的建立及磁小体缺失突变株的筛选,微生物学报,44(4)440~444。

 

专著

  1. 姜伟,曹云鹤主编,《发酵工程实验教程》,科学出版社,2014。

  2. 李颖,关国华主编,《微生物生理学》(参编第二章),2013。

  3. 王颖,王磊,王永星,文莹,田杰生,朱瑞艳,刘枫,关国华,李颖,李季伦,杨光,宋大康,陈芝,陈文峰,姜伟,潘淼(按姓氏笔画),《微生物生理学》(编译第七章:细胞结构与功能;第十四章:氮代谢),高等教育出版社,950000字,2009。


承担项目及课题

  1. 国家自然科学基金面上项目(31570067),OxyR调控磁螺菌MSR-1磁小体 结构和组分变化的分子机理,2016-2019,74.2万元,主持。

  2. 国家自然科学基金面上项目(31170089),MamO和MamE调控磁螺菌MSR-1磁小体晶体形成的机制、2012-2015,62万元,已结题,主持。

  3. 863项目(2006AA02Z233),工业发酵制备细菌纳米磁小体的工艺技术及特殊设备,2006-2008、80万元,已结题,主持。

  4. 北京市自然科学基金(5063045),趋磁细菌纳米磁小体的大量制备及性质,2007/01-12,4万元,已结题,主持。

  5. 国家科技支撑计划(2013BAD10B01-1-07),安全高效饲料添加剂创制与应用,子课题,30万元,参加。

  6. 横向课题,肠道益生菌的发酵技术培训,2017-2018,30万元,主持。

  7. 横向课题,利拉鲁肽三十一肽前体表达菌株发酵培养及产物提取,2017-2019,30万元,主持。

  8. 863项目(2007AA021805),天然纳米磁小体载药体系及其在靶向治疗恶性肿瘤中的应用,2007-2009,260万元,参加。

  9. 国家自然科学基金(30870059),固氮酶还原氮气和质子的分子机制,2009-2011,50万元,参加。

  10. 国家自然科学基金(30570023),磁螺菌磁小体生物合成相关基因的克隆与功能分析, 2006-2009, 30万元,参加。

  11. 973项目,联合固氮菌固氮基因网络调控与酶催化分子机理,2010-2014,214万元,参加。

  12. 现代农业产业技术体系建设项目,食用菌采后贮藏与保鲜,2010-2014,350万元,参加。


专利情况

  1. 姜伟,付刚,李颖,田杰生,用趋磁细菌生产磁性颗粒的方法及其中使用的培养基, 专利号:02125920.8。

  2. 姜伟,郭芳芳,李颖,关国华,田杰生,李季伦, 一种连续、批量纯化细菌磁小体的方法, 专利号:200810223498.2。

  3. 姜伟,刘阳,张扬,李颖,关国华,田杰生,李季伦,一种恒化高密度培养趋磁细菌高产磁小体的方法, 专利号:200810223499.7。

  4. 姜伟,刘阳,郭芳芳,李颖,田杰生,关国华,李季伦, 一种纯化细菌磁小体的系统, 专利号:200820123160.5。

  5. 李颖,姜伟,付刚,李季伦,趋磁细菌纳米磁性颗粒的提取和纯化方法, 专利号:03153488。

  6. 田杰生,刘江宁,姜伟,李颖,李季伦,一种高产纳米磁小体的磁螺菌突变株, 专利号:200610144160.9。

  7. 李颖,李想,姜伟,田杰生,王珍芳,一种细菌纳米磁小体纯度的检测方法, 专利号:200610164835。

  8. 李颖,孙建波,姜伟,田杰生,王珍芳,李季伦,一种载带伯胺基药物的细菌纳米磁小体及其制备方法, 专利号:200710002853.9

  9. 李颖,孙建波,姜伟,田杰生,王珍芳,李季伦,一种培养磁细菌生产磁小体的方法, 专利号:200710177452.7。

  10. 李颖,孙建波,姜伟,田杰生,王珍芳,李季伦,一种培养磁细菌生产磁小体的最适溶氧控制方法,专利号:200710177453.1。

  11. 李颖,唐涛,苗莉莉,姜伟,田杰生,王珍芳,李季伦,一种单细胞微生物的高效破壁方法,专利号:200810055661。

  12. 李颖,李想,王宾,姜伟,田杰生,王珍芳,李季伦,一种基因传递复合物及其制备方法, 专利号:200710002852.4。

  13. 田杰生,李爱华,张昕,张惠媛,李颖,姜伟,王珍芳,一种磁小体抗体复合物及其制备方法和应用, 专利号: 201010112129.3。


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